Riboflavin (Vitamin B2) (E101) β€” What You Need to Know

Safe(10/10)Nature-Identical
10
SAFE
Safety Score: 10/10

What Is Riboflavin (Vitamin B2)?

Riboflavin is vitamin B2, an essential water-soluble nutrient that also serves as a yellow food coloring. It is produced commercially via fermentation using the fungus Ashbya gossypii, yielding a molecule identical to naturally occurring riboflavin. Excess riboflavin is readily excreted in urine, giving it a characteristic bright yellow color.

What Is It Used For?

Used as both a food coloring and a nutritional fortification agent in cereals, baby food, dairy products, and energy drinks. Its dual function as a vitamin and colorant makes it one of the most benign food additives.

Function: Yellow food coloring and vitamin fortification agent
2 mg
Typical daily intake

Safety Assessment

πŸ“Š
Acceptable Daily Intake (ADI)
Not specified mg/kg bw/day
πŸ›οΈ
Regulatory Status
πŸ‡ΊπŸ‡Έ USA (FDA): GRAS (Generally Recognized as Safe)
πŸ‡ͺπŸ‡Ί EU (EFSA): Authorized (ADI not specified β€” no safety concern)
⚠️ Key Concerns

Riboflavin is essentially vitamin B2, an essential nutrient required for normal cellular function, growth, and energy metabolism. No safety concerns exist at any realistic dietary intake level. Excess riboflavin is efficiently excreted via the kidneys, which is why it causes bright yellow urine at higher doses. Both natural extraction and biosynthesis via fermentation using Ashbya gossypii are used in commercial production. EFSA considers it so safe that no numerical ADI was deemed necessary.

Our Assessment

Based on current evidence, Riboflavin (Vitamin B2) is rated Safe with a score of 10/10. This is 4.2 points above the average for colorings (5.8/10). Last reviewed by regulators: 2013.

Commonly Found In

CerealsBaby foodEnergy drinksPastaNutritional supplementsDairy products
Example brands:
Kellogg'sNestlΓ©Red Bull

Always check the label for E101 or 'Riboflavin (Vitamin B2)'

β–ΆChemical Information
Formula: C17H20N4O6
Molecular Weight: 376.36 g/mol
CAS Number: 83-88-5
INS Number: 101
Also known as: E101, Vitamin B2, Lactoflavin, INS 101

Frequently Asked Questions about Riboflavin (Vitamin B2)

Riboflavin (Vitamin B2) has a Safety Score of 10/10 (Safe). Riboflavin is essentially vitamin B2, an essential nutrient required for normal cellular function, growth, and energy metabolism. No safety concerns exist at any realistic dietary intake level. Excess riboflavin is efficiently excreted via the kidneys, which is why it causes bright yellow urine at higher doses. Both natural extraction and biosynthesis via fermentation using Ashbya gossypii are used in commercial production. EFSA considers it so safe that no numerical ADI was deemed necessary.

Quick Facts

E-Number
E101
INS Number
101
Category
Colorings
Origin
Nature-Identical
FDA Status
GRAS (Generally Recognized as Safe)
EFSA Status
Authorized (ADI not specified β€” no safety concern)
ADI
Not specified mg/kg bw/day
Last Review
2013
Dietary Compatibility
Vegan: βœ… YesHalal: βœ… YesKosher: βœ… YesGluten-Free: βœ… Yes
Disclaimer: This information is for educational purposes only and is not medical advice. Always consult a healthcare professional for health-related decisions.

Related Additives

Other colorings in the same family